Coding

Part:BBa_K5250005:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-09-30)


DGC PisoF R240A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 424
    Illegal PstI site found at 493
    Illegal PstI site found at 711
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 424
    Illegal PstI site found at 493
    Illegal PstI site found at 711
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 424
    Illegal PstI site found at 493
    Illegal PstI site found at 711
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 424
    Illegal PstI site found at 493
    Illegal PstI site found at 711
    Illegal NgoMIV site found at 732
    Illegal NgoMIV site found at 855
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for use in italicPseudomonas species IsoF. There is a His-tag attaches for protein purification.


Source

We obtained the sequence from a italicPseudomonas species IsoF gene bank our host lab provided us with.

References